CLASS EUCESTODA

Order Cyclophyllidea

Scolex with 4 suckers and a rostellum that might be armed. Single compact vitellarium. Lateral genital pore. Apolytic proglottid (no uterine pore).

Family Hymenolepidae

Hymenolepis diminuta
Examine the ovum, comparing to Fig. 4.44 of your textbook (page 139). Note the hexacanth larva. The larva is infective to beetles, and develops into a cysticercoid within the hemocoel. The difinitive host is a rat. The eggs of taeniids are similar, but have characteristic radial striations.

Family Taeniidae

Taenia pisiformis
Examine the scolex, noting the suckers and rostellum armed with a double crown of hooks. Compare a mature proglottid to Fig. 4.43D in your textbook (page 138), noting the bilobed ovary, lateral genital pore, and single, compact vitellarium. Examine a gravid proglottid, noting the increased extent of the uterus. Adults are a common tapeworm of dogs and wild canids.

Taenia solium
Examine the scolex, noting the suckers and rostellum armed with hooks. Compare a mature proglottid to Fig. 4.43D in your textbook (page 138), noting the bilobed ovary, lateral genital pore, and single, compact vitellarium. Examine a gravid proglottid, noting the increased extent of the uterus. Adults occur in humans with a swine intermediate host.

Echinococcus granulosus
Examine the whole mount of the cysticerci, and the section of the hydatid cyst, comparing to the endogenous budding pathway illustrated in Fig. 6 (page 146) of Box 4.2 in your textbook. Contrast this with the multilocular cysts of E. multilocularis.

Examine the adult, which is the smallest of the taeniid cestodes, consisting of 3-5 (generally 4) proglottids in addition to the scolex and neck. Compare a mature proglottid to Fig. 4.43D in your textbook (page 138), noting the bilobed ovary, lateral genital pore, and single, compact vitellarium. Examine a gravid proglottid, noting the increased extent of the uterus. The definitive hosts for E. granulosus are domestic and wild canids, and the hydatid cysts occurs in numerous herbivorous mammals, and can infect humans.

Family Anoplocephalidae

Monezia expansa
Examine the scolex and a mature proglottid, noting the characteristic cyclophyllidean morphology. Definitive hosts are sheep, with soil mites serving as intermediate hosts for the cysticercoids.

Order Pseudophyllidea

Scolex with bothria; may have a small number of hooks. Vittelaria and testes diffuse (vitellaria cortical and testes medullary). Genital pore and uterine pore median. Proglottids anapolytic.

Diphyllobothrium latum
Examine the scolex, nothing the bothria (grooves) for attachment. Compare a mature proglottid to Fig. 4.43C of your textbook (page 138). The coracidium of Diphyllobothrium latum infects copepods, developing into a procercoid. Procercoids develop into plerocercoids in fishes, and adults occur in piscivorous mammals (including humans).

Bothriocephalus cuspidatus
Examine the structure of the scolex, and note whatever structures you can locate in the proglottids, comparing to Fig. 4.43C of your textbook (page 138). Bothriocephalus cuspidatus has a similar life cycle to D. latum, but matures in piscivorous fishes.

Triaenophorus nodulosus
Examine the plerocercoid (which has been freed from a cyst), noting the 4 hooks on the scolex. Like B. cuspidatus, T. nodulosus matures in piscivorous fishes.

Order Proteocephalidea

Scolex with 4 suckers; might have an apical organ. Proglottids are similar to those of cyclophyllideans, but vitellaria occur in lateral bands, and proglottids are anapolytic, with eggs being released through breaks in the body wall. Oncospheres develop into plerocercoids in copepods. Aquatic vertebrates can serve as paratenic hosts, and definitive hosts; in some cases the same individual can serve as both.

Ophiotaenia sp.
Examine the scolex noting the 4 suckers. Compare whatever structures you can locate in the proglottids to Fig. 4.43A in your textbook (page 138). This specimen was isolated from the intestine of a bullfrog (Rana catesbeiana).

CLASS MONOGENEA

Subclass Monopisthocotylea

All 3 specimens you will observe are from the family Ancyrocephalidae (within the Order Dactylogyrida) characterized by 2 pairs of eyespots, 14 marginal hooks on the opisthaptor (12 peripheral and 2 central), 2 pairs of hooks (hamuli), and a sclerotized penis with an accessory piece. Compare the specimens of Onchocleidus affinis, O. acer, and O. flieri to Figure 3.1 and compare Actinocleidus bennetti and Clavunculus bifurcatus to Figure 3.2..